Cytogenetics Training for URB and BSMMU Researchers
From 4 – 8 August 2019, Dr. Ashok K. Giri, Ph.D., from the CSIR-Indian Institute of Chemical Biology, in Kolkata, India conducted a hands-on training - “Human Lymphocyte Culture for Chromosomal Aberrations Assay and Micronucleus Assays in Human Lymphocyte Culture and also from Buccal Mucosa Cells and Human Urothelial Cells.” The primarily lab-based training was held at the Pathology Department of Bangabandhu Sheikh Mujib Medical University (BSMMU). Over the course of the week, participants from BSMMU and UChicago Research Bangladesh (URB) learned methods of Human Micronucleus Assay (MN) using lymphocytes, buccal mucosa and urothelial cells. Dr. Giri trained participants on handling slides for MN assay from human buccal mucosa cells and human urothelial cells as part of the training.
Attendees also learned how to harvest a cell aggregate culture after 48 hours of incubation, and practiced microscope reading of MN studies from buccal mucosa cells and urine epithelial cells. Samples from two groups - smokers and non-smokers – were used for this lab observation.Participants are better prepared to use these technologies in bio-medical research following the training, and the capacity building for the study of non-communicable diseases will enhance research throughout Bangladesh.
The training was offered as part of the Bangladesh Center for Research Excellence in Non-Communicable Disease. The training was coordinated by several departments from URB and the venue and lab were provided by BSMMU.